FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4705379
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17500.045.6698043
CCGAGTA19000.043.960022
GAGTAGC45450.043.7239534
AGTAGCT72750.043.148015
CGGAGGT21750.041.3769077
TTGGGAG106950.041.0635386
GTAGCTG74250.040.434026
GCTGGGA137200.039.9860239
TACTCGG20550.037.4900633
CTACTCG20000.037.4418072
CCCGAGT26750.036.8482061
TAGCTGG85200.036.420427
GGAGGCT112850.036.364759
GGGAGGC139650.035.7788058
GGAGGTT57700.034.3134358
CTCGGGA21900.034.18985
TCGGGAG25000.033.406066
AGGCGGA33300.033.0819744
GGCGGAG33750.032.851435
GCTACTC47750.032.63371
Produced by FastQC (version 0.11.5)