FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4705379
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT70950.046.4489365
CGAGTAG18200.045.861613
TTGGGAG107750.045.8109746
CCGAGTA18900.044.1630332
GAGTAGC44100.043.0752754
GCTGGGA139800.041.904289
GTAGCTG75550.041.6207286
CTACTCG20050.041.271112
TACTCGG21100.040.5814173
GGGAGGC139000.040.2297558
CGGAGGT22800.039.7719467
CGTACCG1303.9277802E-738.745153
TAGCTGG86550.038.0005877
GGAGGCT111750.037.9966779
CCCGAGT25800.037.9299161
TGGGAGG141750.035.793757
CTCGGGA22950.035.115435
GCACTTT103500.035.0390971
TTTGGGA115600.034.6082535
AGGCGGA33150.033.8613134
Produced by FastQC (version 0.11.5)