FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4461252
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG14800.036.9738163
TTGGGAG85800.036.75326
CCGAGTA15950.035.6630552
CGGAGGT17000.035.574497
GTAGCTG59800.035.3960236
AGTAGCT61000.034.8177345
GCTGGGA108400.034.603249
GGGAGGC108300.033.8374528
CTACTCG15600.032.770712
GGAGGCT88800.032.3495529
CCCGAGT23550.032.300681
TCGCGTT1801.3758108E-731.9982195
GGCGGAG25100.031.8388655
GAGTAGC38000.031.6428264
AGGCGGA23550.031.4911634
TAGCTGG67950.031.2565387
TACTCGG17300.030.3821953
TGGGAGG108500.030.3245777
CCCGAAT11650.029.8489171
CTCGGGA17500.029.621215
Produced by FastQC (version 0.11.5)