FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5225988
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA19500.041.3537142
CGAGTAG19200.040.8748663
CCCGAGT27900.034.0211831
GAGTAGC45100.032.567524
CTACTCG18450.032.3901372
GCTGGGA125900.032.0809639
CGGAGGT16950.031.4320227
AGTAGCT70450.030.9650615
TACTCGG19300.030.963633
GCTACTC46450.030.2620491
TCGGGAG19700.029.9680126
GGAGGCT102550.029.767379
CTCGGGA19650.029.3114815
CGTACCG1000.00628582328.7999063
GTAGCTG75100.028.2808466
TAGCTGG81900.027.6029857
TCGCGTT2105.30692E-727.4271725
TTGGGAG91100.027.0282576
GGGAGGC117850.026.3915238
CGCGACT1100.00970304926.3481221
Produced by FastQC (version 0.11.5)