FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5225988
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT20150.035.002347
CGAGTAG17950.034.474423
CCGAGTA18350.033.722932
GCTGGGA130100.031.365479
AGTAGCT68950.030.2640255
GAGTAGC44050.029.5662064
TACTCGG19850.029.3621223
CCCGAGT25250.029.3520741
CTACTCG19400.029.3013952
GTAGCTG73600.028.1564316
TTGGGAG92700.028.0214376
GCTACTC45550.027.8027151
GGAGGCT109350.027.774089
CTCGGGA20750.027.3950395
TAGCTGG79150.027.005467
GGGAGGC125750.026.8989858
GGAGGTT56600.026.8294688
AGGCGGA29850.026.51624
GGCGGAG30650.025.8240995
TCGCGTT2259.739779E-725.5841145
Produced by FastQC (version 0.11.5)