FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4560270
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1405.456968E-1251.4258545
CTCGCGT2000.046.801634
CGGAGGT19500.045.4129837
CCGAGTA16800.039.4303782
CGAGTAG16300.039.3142553
GAGTAGC39950.038.0288624
GGCGGAG27650.038.0160755
AGTAGCT68950.038.0081445
TTGGGAG94250.037.812336
GGAGGTT53500.036.8728188
GCTGGGA118150.036.866449
GTAGCTG69100.036.6753436
AGGCGGA27550.036.3279464
TAGCTGG74900.035.5655447
CCCGAGT23700.033.6079371
GCGGAGG25750.033.272036
TACTCGG16450.033.2655943
GGGAGGC117550.033.2572828
GGAGGCT98900.033.1954159
CTACTCG16850.032.476272
Produced by FastQC (version 0.11.5)