FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5123036
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT16750.037.824957
CCGAGTA16350.036.1103822
GCTGGGA109750.034.6369749
TAGCGCG1501.1986649E-633.59835
TACTCGG16000.033.3002663
AGTAGCT61250.032.442445
CGAGTAG17300.032.0464973
GGAGGTT49050.031.2644758
TTGGGAG91900.031.2584846
GGAGGCT95800.031.1884029
GTAGCTG67150.031.0929936
TAGCTGG68900.030.7212337
CTACTCG15500.030.658312
GGGAGGC111000.030.4849458
CTCGGGA17200.029.7194275
TCGGGAG19450.029.61296
GCTACTC41650.029.5521131
CCCGAGT25500.029.2450051
AGGCGGA22950.029.1767044
GGCGGAG25200.029.1413845
Produced by FastQC (version 0.11.5)