FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5123036
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA15600.044.2806322
CGAGTAG15750.042.031463
GCTGGGA116600.037.8955239
AGTAGCT66700.036.2477155
CGTACCG1407.018534E-735.9780163
GAGTAGC41300.035.8909034
CCCGAGT25000.035.6901931
TACTCGG17000.035.1314743
GTAGCTG70950.034.177856
CGGAGGT20950.034.0070727
TAGCTGG76300.033.1998567
TCGGGAG21300.032.0930636
CTCGGGA20650.032.0578965
TAGCGCG1352.2572E-531.9804575
TTGGGAG87500.031.742896
CTACTCG17750.031.2147312
CTACGCG1402.893987E-530.83832
GGAGGCT96850.030.8366039
AGGCGGA26250.030.4271224
GGGAGGC112250.029.5551138
Produced by FastQC (version 0.11.5)