FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4672889
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15000.047.5195663
CCGAGTA16800.047.1429332
CGGAGGT17700.045.556967
GAGTAGC39500.042.105954
AGTAGCT64300.041.988495
GTAGCTG66900.039.1728556
CCCGAGT24300.038.7570461
TTGGGAG102200.038.67516
GCTGGGA122100.037.7965669
GGAGGTT54700.036.0639428
TAGCTGG77900.034.9352877
GGGAGGC119750.034.8107228
GGAGGCT100950.033.733769
GAGGTTG68150.033.0665179
GGCGGAG28500.032.0825465
TACTCGG16200.031.9997123
AGGCGGA28450.031.8872324
TGGGAGG126800.031.0583277
GCGGAGG25800.030.9751386
TCGGGAG19850.030.104236
Produced by FastQC (version 0.11.5)