FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5361609
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG19550.037.5657923
CGAGTAG15950.035.210573
CTACTCG19350.034.9776082
CGGAGGT19100.034.6785747
CCGAGTA16700.034.491872
GCTGGGA125300.032.579079
TCGGGAG23250.032.2045446
CTCGGGA20200.032.077315
TCGCGTT2051.2209057E-831.6078825
AGTAGCT73450.031.562465
GAGTAGC44400.031.4599444
GGCGGAG28050.031.3136545
CTCGCGT2101.542685E-830.8576164
GTAGCTG74150.030.2935526
AGGCGGA29000.030.041844
CCCGAGT24800.029.5151651
GGAGGCT106600.028.9738929
TTGGGAG102750.028.7282286
GCTACTC50250.028.4122561
GGGAGGC124250.028.2188518
Produced by FastQC (version 0.11.5)