FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4153085
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA14200.039.043222
CGAGTAG13600.038.118133
GCTGGGA104200.034.201429
TCGCGTT1102.5394067E-432.7253235
AGTAGCT54150.032.5742385
CTACTCG14250.032.3376432
TACTCGG14100.032.170623
GTAGCTG56400.031.657696
TTGGGAG79550.031.314296
GAGTAGC34950.031.107694
TAGCTGG63900.030.4207257
GGAGGCT85300.030.300669
CGGGTTC12250.029.9737289
GCTACTC37450.028.8165681
CGGAGGT15900.028.5266047
CCCGAGT23700.028.1155971
TCGGGAG16500.027.925616
CTCGGGA15800.027.7958155
GGGAGGC102250.027.5308828
CCGGGTT12900.026.78918
Produced by FastQC (version 0.11.5)