FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4153085
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA13800.040.6702272
TACTCGG15200.038.344453
CTACTCG15050.037.29232
AGTAGCT56000.037.133935
CGAGTAG13550.036.110273
GCTGGGA104850.035.1448759
GTAGCTG59400.034.645016
TTGGGAG80450.033.450816
TCGGGAG19100.032.7753266
GAGTAGC35600.032.541464
CGGAGGT16600.032.5172357
GCTACTC36250.031.7594571
GGGAGGC102850.031.4197358
CCCGAGT20850.031.4048291
TAGCTGG66300.031.1550777
GGAGGCT89850.030.4386929
ACGCGTT950.004901278830.3037839
CTCGGGA18900.029.695725
TCGCGTT2202.440538E-829.4361465
TGGGAGG105500.028.8568137
Produced by FastQC (version 0.11.5)