FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3865923
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGC36700.046.859754
AGTAGCT59600.045.5159075
CGAGTAG14200.044.5925643
CCGAGTA15500.044.566412
GCTGGGA119850.044.316469
TTGGGAG92050.043.1502576
GTAGCTG64800.042.0854846
TACTCGG19300.039.1471443
TAGCTGG74950.038.7932747
GGGAGGC126100.037.7253348
GGAGGCT105300.037.043989
CTACTCG19400.036.7199062
GCACTTT84400.036.5748831
TATCGCG800.002114030335.978093
TGGGAGG124150.035.8251887
CTCGGGA21750.035.399135
TCGGGAG22350.034.770776
CCCGAGT21600.034.645571
GCTACTC43550.033.375771
TTTGGGA97600.033.0290685
Produced by FastQC (version 0.11.5)