FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3178814
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19100.077.652433
CCGAGTA20600.072.6994252
GAGTAGC44100.067.590084
AGTAGCT72950.067.505385
GTAGCTG76400.064.362796
CCCGAGT25800.060.362091
TTGGGAG104500.058.4923326
GCTGGGA148600.057.4125869
TAGCTGG88600.057.2879037
GCACTTT91100.051.9206731
TTTGGGA100500.049.8598185
CAAGTAG34800.049.446993
CCAAGTA37650.048.0002632
ACTTTGG95150.047.822193
GGAGGCT116100.047.8112079
CACTTTG98150.046.6553732
GGGAGGC150950.046.2643178
AAGTAGC38800.045.6482964
TCGGGAG22450.045.5385866
CTTTGGG109700.044.8265464
Produced by FastQC (version 0.11.5)