FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3178814
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT66300.074.5600745
CGAGTAG17400.073.195963
CCGAGTA17750.072.563432
GAGTAGC42000.071.7840354
GTAGCTG72200.067.769586
TTGGGAG105850.060.772876
GCTGGGA146950.059.3550459
CCCGAGT24700.057.0981751
TAGCTGG88500.056.1900447
GCACTTT90350.055.9077571
ACTTTGG93200.053.889313
TTTGGGA104100.053.707315
CACTTTG96050.051.8408162
GGAGGCT118100.051.551949
CAAGTAG33150.050.7920153
CCAAGTA35250.049.1950072
CTACTCG22300.049.0458032
AAGTGCT86850.048.715895
CTTTGGG110200.048.579664
GGGAGGC156750.048.206588
Produced by FastQC (version 0.11.5)