FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4856928
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT19200.041.997257
TACTCGG17600.038.45483
CTACTCG17500.038.2638972
CCGAGTA16250.036.7763822
CGAGTAG16100.035.3294263
AGTAGCT62000.034.952555
TTGGGAG97300.034.850756
TCGCGTT1459.196665E-734.7563485
GAGTAGC36850.034.3882874
GGCGGAG28950.034.070315
GCTGGGA118450.033.9158869
AGGCGGA29100.033.402284
CCCGAGT24000.032.9167671
GTAGCTG63550.032.5146
GGAGGTT53250.032.4485788
GGGAGGC120800.032.0641258
TAGCTGG72050.031.775857
GCTACTC43800.031.7708631
GGAGGCT97000.031.7669899
CTCGGGA20400.031.409715
Produced by FastQC (version 0.11.5)