FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4856928
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT20800.043.9443177
GCTGGGA121950.039.9548239
TACTCGG17200.039.3245123
TTGGGAG101800.039.0878076
CCGAGTA17500.039.061552
CGAGTAG17050.038.8264243
AGGCGGA30950.038.3607644
AGTAGCT66350.037.9569245
GGCGGAG32100.037.658955
GGAGGTT58850.037.0559928
CTACTCG17100.037.0297242
GCTACTC40100.036.9646641
GAGTAGC42600.036.8222924
GGAGGCT102800.035.4258049
GTAGCTG69050.035.222246
GGGAGGC128550.035.1040958
CTCGGGA19050.034.372445
TAGCTGG77500.033.5249257
CCCGAGT24600.033.3452261
GAGGTTG73600.032.1721739
Produced by FastQC (version 0.11.5)