FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5345925
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA21600.046.6672
CGAGTAG19750.045.2053833
TTGGGAG121650.042.551996
AGTAGCT78950.040.3063285
CCCGAGT30200.040.08331
ACGCGAT1452.052002E-839.7244223
GAGTAGC48600.039.259544
GCTGGGA148900.038.0524949
GTAGCTG82900.037.77796
GGGAGGC152550.037.142038
CGGAGGT26450.037.018297
GGAGGCT123650.036.623489
TAGCTGG92900.035.5713277
TACTCGG20900.035.1390083
GGAGGTT68450.034.8142978
TGGGAGG154300.034.7610977
CTACTCG21100.034.4647032
GAGGTTG87550.032.893269
TTTGGGA135600.032.7588465
GCACTTT117350.031.9345931
Produced by FastQC (version 0.11.5)