FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5610648
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT2603.6379788E-1233.2289545
GCTACTC51300.031.781431
GCTGGGA132700.031.3592519
TTGGGAG101500.030.9973226
CTACTCG21000.030.858212
GGAGGCT117650.030.781159
CTCGGGA22900.030.4961535
TACTCGG22450.030.4682163
AGTAGCT74550.030.1311535
TCGGGAG25450.029.9865746
CGAGTAG20350.029.7203983
CCGAGTA21100.029.6882272
CTCGCGT3400.029.6475544
GTAGCTG76300.029.4400716
GGGAGGC132750.029.2323028
CCCGAGT30700.028.0877461
GAGTAGC52300.028.0845994
CCCGAAT14400.027.6763291
CGGAGGT19300.027.604717
TAGCTGG84700.027.2854067
Produced by FastQC (version 0.11.5)