FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4525770
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA16850.033.3299562
CGAGTAG16900.033.231353
CGGAGGT17800.032.762447
GCTACTC40000.031.8841651
GCTGGGA107500.031.8123999
GAGTAGC39050.031.713744
AGTAGCT59650.030.8987395
CTACTCG16100.030.8576772
TTGGGAG82600.029.8968436
TACTCGG16950.029.3102443
GGAGGTT47750.028.0447038
GGAGGCT95900.027.627439
GTAGCTG63850.027.4003856
TCGGGAG20050.026.5722666
CTCGGGA19050.026.4553995
TAGCTGG69800.025.7867057
GAGGTTG58000.025.5711659
GGGAGGC110900.025.124118
GTGCTGG70850.024.4899837
TGGGAGG108250.024.408977
Produced by FastQC (version 0.11.5)