FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4525770
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1704.1836756E-1142.3269925
CGAGTAG14300.041.76463
CCGAGTA15550.040.2582742
CTCGGGA20100.035.4409565
TACTCGG18550.035.299113
CTACTCG17800.035.169452
GAGTAGC36000.034.7786754
AGTAGCT59100.034.2125245
GCTGGGA111950.033.943219
TCGGGAG21900.033.8422626
GGAGGCT97850.032.0677649
GTAGCTG63100.031.929716
TTGGGAG86950.031.8608576
CCCGAGT24500.031.7193281
TAGCTGG69850.031.1159887
GCTACTC41000.030.5373731
GGGAGGC116800.030.130698
CGCGTTG2151.9514118E-830.1210656
CGGAGGT20200.029.5712577
GGAGGTT52350.029.419838
Produced by FastQC (version 0.11.5)