FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4690573
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18500.053.7088133
CCGAGTA19100.053.5300672
GAGTAGC48600.049.630284
AGTAGCT78150.047.8122835
GTAGCTG79200.046.1784826
TTGGGAG113200.044.2653586
CCCGAGT26350.044.259571
GCTGGGA152800.043.630369
CGGAGGT26050.042.561237
TAGCTGG94100.041.085247
TACTCGG20550.040.6428683
GGAGGCT127350.038.7816549
GGGAGGC152650.037.683498
CTACTCG21500.037.1729772
GGAGGTT63550.037.158618
TCGGGAG24900.034.696296
GCTACTC53400.034.3195231
TTTGGGA119100.034.0932585
TGGGAGG156750.033.8501857
GCACTTT100900.033.5982481
Produced by FastQC (version 0.11.5)