FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3797837
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT63550.050.5002635
CCGAGTA15500.049.6736872
GAGTAGC40350.049.2198074
GCTGGGA130850.048.180739
CGAGTAG15950.047.369953
GTAGCTG67350.047.2235876
TTGGGAG98950.046.0322536
TAGCTGG75650.043.8565987
GGAGGCT112850.042.4732639
CTACTCG17250.042.1314742
TACTCGG17700.040.653793
CCCGAGT21450.040.5912481
GGGAGGC138550.040.516428
GCTACTC42200.040.4119871
GCACTTT86300.037.8546641
AAGTGCT85800.037.655935
TGGGAGG133400.037.4948777
TTTGGGA99000.036.6327635
TCGGGAG22100.036.4670036
CGGAGGT20850.036.243177
Produced by FastQC (version 0.11.5)