FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5836925
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA22200.034.0558622
CGAGTAG21350.032.3755953
CGGAGGT21200.031.2431957
TACTCGG20250.030.5786153
CTACTCG19350.030.1411342
GCTGGGA139250.029.8838189
AGTAGCT75800.029.63395
TCGGGAG22500.028.7980756
CCCGAGT33400.028.6524851
CTCGCGT3650.027.6171974
CTCGGGA22700.027.5928695
GTAGCTG81100.027.519746
GGAGGTT58350.027.514878
GAGTAGC51250.027.255384
GGCGGAG31900.026.8571385
GCTACTC51300.026.8516581
GGAGGCT116700.026.5277889
TAGCTGG92300.025.3504187
GCGGAGG28350.024.3793246
AGGCGGA33600.024.214954
Produced by FastQC (version 0.11.5)