FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5836925
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG21850.035.8949662
TACTCGG23300.034.8964273
CGAGTAG18400.033.239893
CCGAGTA19350.033.095392
CTCGGGA24000.032.9791835
TCGGGAG26450.032.372766
CGGAGGT25500.031.3275787
GCTGGGA140500.031.1959
GCTACTC51350.029.8467881
AGTAGCT75850.029.3130725
GAGTAGC49950.029.2428044
TCGCGTT2303.7596692E-828.1561435
GTAGCTG81000.028.071176
GGAGGTT63750.027.8843658
TTGGGAG111250.027.746986
AGGCGGA33600.027.625424
TAGCTGG87400.027.4205937
GGAGGCT121050.027.289279
GGCGGAG36300.026.759975
GGGAGGC144250.026.1931368
Produced by FastQC (version 0.11.5)