FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5064360
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT2250.051.197115
CTCGCGT2850.042.9483384
CGCGTTG2850.040.4187746
ACTCGCG3000.036.0008133
CGGAGGT18350.034.526667
CCGAGTA19100.034.3047752
GCTGGGA123700.034.281019
AGTAGCT71300.033.927965
GTAGCTG71100.033.7196166
CGAGTAG18200.032.044683
GAGTAGC45150.031.8944074
TTGGGAG93350.031.5440166
TAGCTGG81400.030.779597
CCCGAGT27150.030.6906531
TACTCGG16700.030.1803233
GGAGGTT48650.029.8934828
GGAGGCT101500.029.8623529
CTACTCG16400.028.5375122
GGGAGGC115800.028.2263858
AGGCGGA26800.027.6722664
Produced by FastQC (version 0.11.5)