FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5064360
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA16500.040.992852
CGGAGGT21400.039.347177
CGAGTAG15800.038.2548453
AGTAGCT65350.036.00535
GCTGGGA123900.035.3161779
TTGGGAG97000.034.197436
CCCGAGT25350.034.0617941
GTAGCTG69350.033.7210546
GAGTAGC42850.032.9131554
GGGAGGC124750.032.0756658
GGAGGCT107350.031.2410289
GGAGGTT58550.031.2211048
TACTCGG19150.031.1869983
CTACTCG18250.031.1478792
TAGCTGG82450.030.2886737
GCGGAGG27850.029.9707136
GGCGGAG30450.029.53845
CTCGGGA19700.029.2205245
GAGGTTG74150.028.72919
GCTACTC45150.028.6865981
Produced by FastQC (version 0.11.5)