FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6153476
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG23700.029.4755952
CCGAGTA27400.028.6493762
TACTCGG25400.028.3497353
AGTAGCT102200.028.1089575
CGAGTAG27100.027.368483
GCTGGGA195550.027.171989
GAGTAGC64500.025.9002514
GTAGCTG111800.025.6953076
TAGCTGG120550.025.323367
CCCGAGT39750.023.7760681
GCTACTC62550.022.6065671
TTGGGAG133150.021.6833296
CTCGGGA34350.021.169835
AAGTGCT127300.020.1912215
CCCAAAG160500.020.0477521
TCGGGAG35250.020.0165736
GGAGGCT153600.019.8276799
AAAGTGC130700.019.7234614
GTGCTGG131200.019.7007777
CTACTTG50800.019.5638622
Produced by FastQC (version 0.11.5)