FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6153476
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24300.035.5552023
CCGAGTA25850.033.4232672
GAGTAGC60950.029.8864964
AGTAGCT92100.028.4557445
CCCGAGT36350.027.1403181
GCTGGGA189050.026.9259419
GTAGCTG99000.025.527026
TAGCTGG111350.025.4761717
TTGGGAG132950.024.2073576
CTACTCG30150.024.1191642
GCTACTC65500.024.0769541
TACTCGG28850.023.7086033
GGAGGCT160600.020.5776279
GGGAGGC188950.020.3099358
CCCAAAG155250.019.0637781
CCAAAGT140200.018.9498842
AGCTGGG164750.018.9230298
AAGTGCT127700.018.5495435
CTCGGGA36950.018.5113185
TCGGGAG37050.018.4613556
Produced by FastQC (version 0.11.5)