FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4524443
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22450.068.3094943
CCGAGTA23300.068.289542
AGTAGCT86150.067.526455
GAGTAGC56100.063.9122774
GTAGCTG92300.061.7790646
TTGGGAG134900.058.3879476
GCTGGGA189650.056.7173049
CCCGAGT28400.056.286631
TAGCTGG108900.054.874187
TACTCGG25400.054.423363
CTACTCG25300.050.6544152
GGAGGCT151750.050.2441029
TCGGGAG29950.049.040086
GCACTTT119750.048.9462621
GCTACTC61150.048.043471
GGGAGGC193700.047.948828
TTTGGGA134350.047.8554735
CTCGGGA30250.047.6016965
TGGGAGG188300.045.2326747
AAGTGCT114150.045.097035
Produced by FastQC (version 0.11.5)