FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4647362
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA18400.056.7496832
CGAGTAG18250.055.631763
AGTAGCT71450.050.2831885
GAGTAGC43300.049.3874244
GTAGCTG76450.047.1829076
GCTGGGA147150.045.3080339
CCCGAGT25500.043.5555881
TAGCTGG88700.043.3453067
TTGGGAG108100.042.826226
CGGAGGT24300.041.1844257
GGAGGCT116600.038.4692739
GGAGGTT62350.036.9520078
GGGAGGC145100.036.8676838
TACTCGG19100.036.191323
TGGGAGG144750.034.768277
GCTACTC48050.034.071861
GAGGTTG77650.033.565389
AGGCGGA33250.033.348554
CTACTCG19000.032.9745522
GTGCTGG100100.032.654747
Produced by FastQC (version 0.11.5)