FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4751509
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT16050.034.990067
GGAGGCT91600.030.1042889
TTGGGAG80900.029.4580866
AGGCGGA23800.027.531554
AGTAGCT59850.027.4281025
GCTGGGA110950.027.384849
GGGAGGC104400.027.1029878
GAGTAGC38750.026.9440294
GGCGGAG24350.026.3157715
CGAGTAG15050.026.3146113
GGAGGTT46800.026.3072438
CCCGAAT9650.026.1606161
GCTACTC41950.025.7909051
CTACTCG17100.025.6894472
CCGAGTA15650.025.3086742
TACTCGG17150.025.1917273
GTAGCTG64100.025.0479346
CTCGCGT2301.172446E-625.0454224
GCGGAGG22150.025.0289176
CCCGAGT21650.024.6536431
Produced by FastQC (version 0.11.5)