FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4751509
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA16300.042.8457642
CGAGTAG16000.042.7491463
CTACTCG16900.037.490372
GAGTAGC39400.037.278444
TACTCGG17400.036.4130673
AGTAGCT60400.036.3568885
CCCGAGT22350.034.472371
CTCGGGA18850.034.375975
GCTGGGA114650.033.4716389
TCGGGAG21600.032.999346
TTGGGAG89650.032.365226
GTAGCTG68200.032.3043376
CGGAGGT18750.032.2553567
GCTACTC41100.031.53521
TAGCTGG72350.031.0484437
GGAGGCT99800.030.9492829
GGAGGTT50300.030.917878
GGGAGGC114150.030.7168648
AGGCGGA23500.030.3313084
ACTCGGG23800.028.7389224
Produced by FastQC (version 0.11.5)