FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5679223
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT23300.041.0971537
CGAGTAG19000.040.1668973
AGTAGCT77050.038.1244475
CCGAGTA19850.037.3587842
GCTGGGA152500.037.013689
TACTCGG20250.036.265293
TTGGGAG117550.036.197696
CTACTCG20000.034.9186742
GAGTAGC50550.034.7523884
CCCGAGT26650.034.3143731
GGAGGCT121250.033.7867629
GTAGCTG82950.033.7636386
GGGAGGC141900.033.233418
AGGCGGA34650.033.037714
GGAGGTT68150.032.961338
TAGCTGG90000.032.878757
CTCGGGA21950.032.4725725
GCTACTC50550.031.0530931
GGCGGAG37600.030.2541715
TGGGAGG152750.029.6473227
Produced by FastQC (version 0.11.5)