FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5343382
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA17400.031.8710042
TACTCGG19100.031.6690623
CGAGTAG17400.030.6246913
CGGAGGT23100.030.2331897
GCTACTC48600.029.0928381
GCTGGGA128600.028.9450159
TCGGGAG24150.028.6205736
CTACTCG20250.028.4524232
AGTAGCT70900.028.0276765
GGAGGCT116050.027.6703059
GGAGGTT60550.026.8731468
TTGGGAG107500.026.455316
GAGTAGC45850.026.0707764
TAGCTGG79400.026.0246947
GTAGCTG75300.025.5293946
CCCGAGT26450.025.3643381
CTCGGGA23650.025.2680175
AGGCGGA31100.025.2377874
GGGAGGC139100.025.1555238
GAGGTTG76450.024.1094179
Produced by FastQC (version 0.11.5)