FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5343382
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG19200.040.874513
CCGAGTA18250.040.6351282
CTACTCG19250.039.646282
CGAGTAG18850.038.577793
TCGGGAG24950.035.2059946
CCCGAGT25350.034.9377631
CGGAGGT23350.032.6848347
GAGTAGC46250.032.5358284
CTCGGGA23550.032.4072575
GCTACTC49400.032.3589171
TTGGGAG106350.031.6159826
AGTAGCT72350.031.2477255
GGGAGGC138950.031.1935838
GCTGGGA142500.030.4670039
GGAGGCT117300.029.9536089
GTAGCTG77350.028.7624236
GGAGGTT59700.028.461978
AGGCGGA32550.028.3130264
TAGCTGG82500.028.2760267
TCGCGTT2054.2974898E-728.0972275
Produced by FastQC (version 0.11.5)