FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4731432
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1501.0913936E-1147.999055
CGGAGGT19250.038.1498957
TTGGGAG91600.033.6412546
CCGAGTA15850.033.627272
AGGCGGA25200.033.434274
CGAGTAG14700.031.8424973
TACTCGG16750.031.8147143
GCTGGGA121300.031.8147059
GGAGGCT99300.031.1776289
AGTAGCT63350.030.9133595
GGAGGTT53850.030.751488
GGCGGAG29050.030.4847645
CTACTCG17850.029.4562
GGGAGGC119750.029.3405468
GAGTAGC40050.029.128564
GCTACTC43600.028.622991
TAGCTGG71200.028.5162897
TAGCGGG4050.028.443885
TGGGAGG120050.028.3076467
GTAGCTG68050.028.0376516
Produced by FastQC (version 0.11.5)