FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5051218
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16550.048.296733
CCGAGTA18550.046.9789162
GAGTAGC42400.038.7200554
TACTCGG16650.038.4918253
AGTAGCT66100.037.5786865
CTACTCG17950.036.512242
GCTGGGA132800.035.511359
CCCGAGT27150.034.2757721
TAGCTGG76250.034.1816257
GTAGCTG73700.034.0943076
TTGGGAG95300.032.4862446
CGGAGGT19750.032.444937
CTCGGGA19350.032.3714565
GCTACTC46100.032.2354471
GGGAGGC127800.030.8726338
CGTACCG1402.8791992E-530.8614253
GGAGGCT109000.030.5828849
GGAGGTT54500.029.8562938
TCGGGAG22800.028.4204946
AGGCGGA27850.027.1475734
Produced by FastQC (version 0.11.5)