FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5561870
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA20200.042.0588872
CGAGTAG20500.040.038533
AGTAGCT75000.038.879525
GAGTAGC46800.038.4610634
TTGGGAG113550.037.0933346
GCTGGGA149850.035.4590239
CCCGAGT28950.035.0722851
CGGAGGT26500.034.5052347
GTAGCTG82700.033.6053926
GGGAGGC153700.033.16558
TAGCTGG87650.032.775417
GGAGGCT134750.032.3261349
TGGGAGG152000.031.4996137
GCTACTC58100.030.9854241
GGAGGTT67850.030.6672738
TACTCGG24000.030.5996253
CTACTCG25200.029.713922
TTTGGGA123800.028.613545
GCACTTT113050.028.0906371
AGGCGGA36350.026.5416284
Produced by FastQC (version 0.11.5)