FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5334396
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA19150.036.8450242
GCTGGGA128100.035.577569
CGGAGGT21500.033.8224037
CGAGTAG19200.033.749153
AGTAGCT70900.032.901855
GGAGGTT59550.031.1931698
GAGTAGC44100.031.182894
CCCGAGT26100.031.176011
AGGCGGA27900.030.9669634
GTAGCTG74400.030.8701926
CTACTCG18950.029.6351382
GAGGTTG75600.029.0468889
TAGCTGG83800.028.7820917
TACTCGG19200.028.4992833
TTGGGAG97700.028.2981626
GGCGGAG30950.027.915295
GCTACTC46550.027.0707951
GCGGAGG29300.027.0300356
GTGCTGG89350.025.4632647
CTCGGGA21600.025.3326955
Produced by FastQC (version 0.11.5)