FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5694261
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA22350.039.3016972
CGAGTAG22350.038.9795533
GAGTAGC53000.037.358194
AGTAGCT80600.037.071665
TACTCGG23750.035.469193
GCTGGGA167250.034.8266229
TTGGGAG124500.034.814186
CGGAGGT28250.033.6422047
CTACTCG23700.033.4174542
CCCGAGT30600.033.4156041
GTAGCTG90300.032.8502356
TAGCTGG100550.032.2225157
GGAGGCT142900.031.842999
GGGAGGC169300.031.0877598
TGGGAGG169150.028.8167957
GGAGGTT68000.027.4233078
TCGGGAG32100.027.3642656
CTCGGGA31300.026.4534595
AGGCGGA38650.025.8937114
GCACTTT112200.025.222151
Produced by FastQC (version 0.11.5)