FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4818348
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT16050.039.0272647
CCGAGTA15650.038.1956442
CGAGTAG15250.037.303433
CCCGAGT22950.033.319591
TTGGGAG88300.032.370826
TACTCGG15350.032.3692173
AGTAGCT62450.031.7047355
GCTGGGA116650.031.3547159
AGGCGGA25500.030.7806514
GAGTAGC39950.030.4622124
GGCGGAG26000.029.6301825
CTACTCG16200.029.3412762
GTAGCTG68500.029.219866
GGGAGGC110650.028.9555958
GGAGGCT95750.028.4986539
TAGCTGG73500.027.9178167
CTCGGGA17200.027.627365
GGAGGTT52650.027.6233848
GCTACTC39550.027.5427281
TGGGAGG113750.027.3436367
Produced by FastQC (version 0.11.5)