FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4867587
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA25900.066.717642
CGAGTAG25800.066.697173
AGTAGCT100650.065.740195
GAGTAGC64550.063.8012244
GTAGCTG109400.060.416366
TTGGGAG160900.057.6353656
GCTGGGA226350.054.6158879
TAGCTGG126100.053.8425757
TACTCGG32100.052.2612883
GCACTTT132700.051.6577381
CCCGAGT35300.050.5878641
GGAGGCT177250.049.9629559
CTACTCG33000.049.745082
TTTGGGA154100.049.2922975
ACTTTGG140100.047.8968543
GGGAGGC232150.047.451728
CACTTTG142250.047.172932
GCTACTC74200.046.8719021
AAGTGCT128900.046.026035
TCGGGAG40650.043.925876
Produced by FastQC (version 0.11.5)