FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4948814
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG23150.051.016813
CTACTCG22850.049.4869772
AGTAGCT77650.045.619195
CGAGTAG20100.044.785193
TTGGGAG116000.044.3784336
GAGTAGC49700.044.0487174
TCGGGAG28150.043.7362566
CCGAGTA21150.043.248482
GCTGGGA162200.041.059619
CTCGGGA27950.040.7004475
GTAGCTG85300.039.7553676
GGAGGCT133500.039.1542859
GCTACTC56800.038.222141
GGGAGGC171600.037.5517048
ACTCGGG31950.036.9648554
TAGCTGG95200.036.9068347
CCCGAGT27850.036.5166361
CGGAGGT27400.034.4226767
TTTGGGA124400.033.3948365
TGGGAGG167950.033.137787
Produced by FastQC (version 0.11.5)