FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4948814
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA19650.054.2276652
CGAGTAG18950.053.571233
GAGTAGC50650.047.904044
AGTAGCT78400.047.294755
TTGGGAG127550.046.3994756
GTAGCTG82550.044.9171186
TACTCGG25200.044.5703243
CTACTCG24500.044.3744132
CCCGAGT27400.043.8896641
GGAGGCT143300.043.1084949
GCTGGGA169750.043.0080649
TAGCTGG94250.040.9454047
CTCGGGA29500.039.0498855
GCTACTC59100.038.868661
GGGAGGC176100.038.6770678
CGGAGGT31000.037.3926247
TCGGGAG30500.037.2974366
GCACTTT109950.036.283641
TGGGAGG175950.036.0093357
TTTGGGA129850.035.92985
Produced by FastQC (version 0.11.5)