FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5624976
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21650.054.2115483
CCGAGTA24250.052.8620152
GAGTAGC57600.046.5023464
AGTAGCT89950.045.784495
CCCGAGT31100.042.9099431
GTAGCTG95050.042.797646
GCTGGGA176250.041.9940269
TTGGGAG138600.041.1420066
TAGCTGG106200.040.8127177
TACTCGG25000.039.1706733
CGGAGGT27750.037.6208427
GGAGGCT143450.037.0909049
GGGAGGC176350.037.070978
GGAGGTT74000.036.291148
CTACTCG25950.036.077922
GCTACTC61050.036.0380061
CTCGGGA29500.033.6806565
TGGGAGG184150.033.545877
TCGGGAG31200.032.076266
GCACTTT124500.032.040731
Produced by FastQC (version 0.11.5)