FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5624976
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA23050.051.5390132
CGAGTAG24000.049.1989363
TTGGGAG141050.047.93096
GAGTAGC55550.046.789274
AGTAGCT87350.045.993285
GCTGGGA179550.044.750919
TACTCGG25200.043.999053
CTACTCG23750.043.956942
GTAGCTG92250.043.940516
CGGAGGT30550.042.4213377
GGAGGCT153100.042.3243649
CCCGAGT31800.040.759271
GGGAGGC195300.040.367798
TAGCTGG104800.040.327377
GCTACTC61750.040.2312161
TCGGGAG31400.038.2921646
TGGGAGG193550.037.384887
CTCGGGA29700.037.0901035
GCACTTT130050.037.04221
TTTGGGA151850.036.9356465
Produced by FastQC (version 0.11.5)