FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5300719
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA19700.038.3865052
CGAGTAG18550.038.042053
TTGGGAG108550.037.0771526
AGTAGCT74000.036.874995
TACTCGG19300.036.5637323
GCTGGGA138150.036.1687059
GAGTAGC47150.035.1239474
GGAGGCT116200.034.0785379
GGGAGGC135500.033.9004758
CTACTCG20100.033.322872
GTAGCTG81050.033.312156
CCCGAGT27150.033.211521
CGGAGGT21950.032.8012127
GCTACTC48700.032.29061
TCGGGAG25450.032.2508746
TAGCTGG86650.032.1563537
CTCGGGA23150.032.033965
TGGGAGG144750.029.2471227
AGGCGGA29850.028.4639284
TACGACG1306.755219E-427.6917925
Produced by FastQC (version 0.11.5)