FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5300719
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA17450.042.9106252
CGAGTAG17600.042.544913
GAGTAGC44300.039.3313264
GCTGGGA142850.038.2046249
AGTAGCT72950.037.899455
TTGGGAG114000.037.1363686
CGGAGGT23100.036.4670687
GTAGCTG80000.034.469566
TAGCTGG86700.034.0480087
AGGCGGA31950.033.3516854
GGGAGGC140400.032.9226538
GGAGGCT115350.032.0203979
CCCGAGT25650.032.0032121
TGGGAGG143150.031.384867
GGAGGTT62750.031.2091628
GGCGGAG36350.028.9184765
GAGGTTG79350.028.6726029
TACTCGG21900.028.2736113
TCGGGAG24800.028.160936
TTTGGGA124800.027.922725
Produced by FastQC (version 0.11.5)