FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5510062
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA19000.048.8956032
CGAGTAG19150.046.6280443
AGTAGCT80550.040.1332325
GAGTAGC49900.039.54074
GCTGGGA153100.038.2330329
TTGGGAG125100.037.697056
CCCGAGT28000.037.6110081
GTAGCTG83900.036.9861076
CGGAGGT24650.036.2183047
TAGCTGG92550.035.3185427
TACTCGG21150.034.0473983
CTCGGGA23200.033.8268745
GGAGGCT129300.033.0201879
GGGAGGC160650.032.8956688
GGAGGTT66350.032.445448
CTACTCG21450.031.8955442
AGGCGGA36500.031.5661354
GCTACTC55300.030.913161
TGGGAGG162750.030.1707947
TTTGGGA134950.029.6103445
Produced by FastQC (version 0.11.5)